56G Oncology Accel-Amplicon® Panel v2

All-in-One Oncology Panel with Sample Identification

User Manual Brochure

Product Description

The Accel-Amplicon 56G Oncology Panel v2 offers comprehensive and hotspot coverage of 56 clinically-relevant oncology-related genes. This panel utilizes a 263-amplicon design, covering over 16,000 COSMIC mutations (Forbes et al. Oxford Journals. 2014), to generate targeted libraries compatible with Illumina sequencing platforms and now includes 104 exonic and gender Sample_ID amplicons spiked in at a low percentage (2-4% of reads) for tracking tumor-normal pairs and samples in longitudinal studies.

The Accel-Amplicon 56G Oncology Panel v2 is compatible with short DNA fragments from both FFPE and cfDNA samples, and is well-suited for detection of clinically-relevant allele variants in DNA from circulating tumor cells (CTCs). This product is a complete kit that includes all components necessary for generating ready-to-sequence libraries, including primer pairs and indexed sequencing adapters.

  • Single-tube assay to interrogate 56G and Sample_ID targets
  • Ready-to-sequence libraries in 2 hours
  • Inputs as low as 10 ng
  • On-target specificity and coverage uniformity > 95%
  • Average amplicon size of 138 bp for compatibility with cfDNA and FFPE
  • Limit of detection as low as 1%
  • Leverage a power of discrimination over 1 in 100,000
  • Easily track samples within and between studies
  • Validate tumor/normal pairs and track samples throughout longitudinal studies
  • Compliment WGS or exome sequencing for sample tracking
  • Leverages the high fidelity performance of the Illumina platform
  • Complete library generation in a single kit

Decrease Input, Not Sensitivity

Like the Accel-Amplicon Comprehensive TP53 Panel, the Accel-Amplicon 56G Oncology Panel v2 offers high sensitivity variant detection from input amounts from 10-25 ng. The kit utilizes Illumina-compatible dual-indexed adapter sequences and has been validated on the MiSeq® and HiSeq® platforms.

Workflow

The Accel-Amplicon workflow uses a fast, single-tube approach consisting of a 90-minute target enrichment amplification step and a 10-minute adapter ligation step, yielding a 2-hour start-to-finish procedure.

Single Tube Workflow2

Product Specifications

Genes Represented in the 56G Oncology Panel and Number of Amplicons

Performance Data

The data below demonstrate the performance of the Accel-Amplicon 56G Oncology Panel on Illumina Platform for a variety of sample types.

High Coverage Uniformity Across Sample Types

Reproducible Variant Calling from Q-Seq HDx™ Quantitative Standards

Sequencing data files (FASTQ, BED, BAM, and VCF) for this amplicon panel are available on request.

  • Catalog Number
    AL-56248-SWI
  • Supplier
    Swift Biosciences
  • Size
  • Shipping
    Dry Ice/RT
  • Price
  • 4.444,00 €
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you need any help?

Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com