SARS-CoV-2 Swift Amplicon® Panel
Whole viral genome single-tube NGS assayUser Manual Brochure
The Swift Amplicon® SARS-CoV-2 Panel offers a robust NGS workflow that provides optimal coverage and NGS data quality on Illumina® sequencing platforms. This kit leverages patented multiplex PCR technology, enabling library construction from 1 st-strand or 2nd-strand cDNA using tiled primer pairs to target the entire 29.9 kb viral genome with a single pool of multiplexed primer pairs.
Primers were designed against the NCBI Reference Sequence NC_045512.2 (Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome). In silico analysis predicted zero off-target products from human host genome sequences.Whole viral genome single-tube NGS assay providing optimal SARS-CoV-2 coverage and NGS data quality
- 341 amplicons sized 116-255 bp (average 150 bp)
- 98% SARS-CoV-2 genome coverage (29,313 of 29,903 bases)
- 2 hours cDNA-to-library processing time