SensiFAST HRM Kit

Precise, highly reproducible and fast genotyping of sequence variants through high resolution melting.

  • Accurate – clear discrimination of even the most challenging sequence differences
  • Reproducible – unparalleled consistency between replicate difference plots for increased confidence in sample characterization
  • Sensitive – efficient detection from even very limiting amounts of sample
  • Flexible – enables reliable characterization of a broad range of sequence differences
  • Fast – rapid PCR amplification prior to high resolution melting, enabling higher throughput
user manual

Product Description

SensiFAST™ HRM Kit has been developed for detailed characterization of samples according to their base composition, length and GC content by high resolution melting. The latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensure the SensiFAST HRM Kit delivers reproducible, accurate HRM analysis.

SensiFAST HRM Kit contains EvaGreen®, a third generation, saturating fluorescent dye which selectively binds to double-stranded DNA. In contrast to dyes such as SYBR® Green I, EvaGreen can be used at higher concentrations without inhibiting PCR and shows equal binding affinity for GC-rich and AT-rich regions. The combination of our SensiFAST DNA Polymerase, unique buffer system and EvaGreen dye enables amplification and discrimination of even the most challenging sequence differences, such as class IV SNPs without sequence preference. Since it does not require expensive labelled oligonucleotide probes, SensiFAST HRM Kit is also a cost effective alternative to traditional probe based genotyping methods.

Applications

  • SNP genotyping
  • Characterization of haplotype blocks
  • DNA mapping
  • Detection of insertions, deletions and translocations
  • DNA fingerprinting
  • DNA methylation analysis
  • Gene scanning
  • Species identification
  • Allelic prevalence population studies
  • Screening for loss of heterozygosity

Highly successful genotyping of a Class 4 (A/T) SNP using SensiFAST HRM

The three different genotypes of an A>T SNP were analysed on the Rotor-Gene 6000, using SensiFAST HRM Kit. A difference plot of this data illustrates the effect of subtracting an average heterozygous curve from all curves and plotting the results.

  • Price
  • 392,00 €
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you need any help?

Please contact:

Dr. Monika Haas

Tel. +49 (0) 6221 714 15 16 info@biocat.com