Tetro Reverse Transcriptase Enzyme
Tetro™ Reverse Transcriptase is a highly sensitive, high stability MMLV reverse transcriptase. Tetro Reverse Transcriptase is optimized for reverse transcription reactions using a wide range of total RNA amounts, such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis.Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, cDNA library construction, and the production of templates for RT-PCR analysis of gene expression.
- Highly sensitive - for high-quality, full length cDNA from as little as 10 pg of total RNA
- Ultra-stable - for long genes and rare transcripts
- High yield - produces high quality cDNA ideal for PCR
- Broad dynamic range - 10 pg to 2 μg of RNA
Tetro™ Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor (fig. 1), with highly efficient and sensitive transcription, from as little as 10 pg, up to 2 μg of RNA (fig. 2).
Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures.
Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.
High sensitivity of Tetro Reverse Transcriptase on mouse total RNA