Tetro Reverse Transcriptase Enzyme

Tetro™ Reverse Transcriptase is a highly sensitive, high stability MMLV reverse transcriptase. Tetro Reverse Transcriptase is optimized for reverse transcription reactions using a wide range of total RNA amounts, such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis.

Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, cDNA library construction, and the production of templates for RT-PCR analysis of gene expression.

  • Highly sensitive - for high-quality, full length cDNA from as little as 10 pg of total RNA
  • Ultra-stable - for long genes and rare transcripts
  • High yield - produces high quality cDNA ideal for PCR
  • Broad dynamic range - 10 pg to 2 μg of RNA
user manual

Product Description

Tetro™ Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor (fig. 1), with highly efficient and sensitive transcription, from as little as 10 pg, up to 2 μg of RNA (fig. 2).

Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures.

Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.

High sensitivity of Tetro Reverse Transcriptase on mouse total RNA

Fig.1. A five-fold serial dilution of total RNA from mouse brain (1 μg to 10 pg) was reverse transcribed using 50 Units of Tetro Reverse Transcriptase, oligo (dT)18 abd random hexamers. The resultant cDNA was then used as template in a PCR using primers for amplification of a 700 bp fragment from mouse b-actin. Lanes 1-5 correspond to PCR product from the serial dilution above, reactions were carried out in duplicate. HyperLadder 50bp (M).

High sensitivity on human DNA

Tetroreverse2 550 C2

Fig.2. A ten-fold serial dilution of human total RNA (1 μg to 10 pg) was reverse transcribed using Tetro Reverse Transcriptase and oligo (dT)18. The resultant cDNA was then used as a template in a PCR using primers for amplification of a 470 bp fragment from human GADPH. Lanes 1-5 correspond to PCR from the serial dilution above, reactions were carried out in duplicate. HyperLadder 50bp (M).

  • Catalog Number
    BIO-65050-BL
  • Supplier
    Bioline
  • Size
  • Shipping
    Blue Ice
  • Price
  • 175,00 €
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