SensiFAST SYBR Hi-ROX Kit

Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.

  • Reproducible – consistent results between technical replicates for increased confidence in results
  • Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
  • Sensitive – reliable quantification of low abundance targets and scarce samples
  • Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
  • Fast – delivers reproducible, accurate assay results in as little as 30 minutes
user manual One-step vs. Two-step real-time RT PCR

Product Description

The SensiFAST™ SYBR Hi-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX.

A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR Hi-ROX Kit produces reliable assay results under fast thermal cycling conditions. An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.

The SensiFAST SYBR® Hi-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications

  • Gene expression analysis
  • DNA / RNA target detection
  • miRNA profiling / quantification
  • Copy number variation (CNV) analysis

SensiFAST SYBR using fast cycling conditions

A fragment of ubiquitin gene was amplified using SensiFAST SYBR Hi-ROX (blue) and the results were compared with amplifications using a Kit from Supplier Q (red). The process used a 10 fold serial dilution of human cDNA (in quadruplicate) over four orders of magnitude. The results illustrate SensiFAST SYBR Hi-ROX was faster (earlier Ct), with higher specificity and reproducibility.

SensiFAST Products Customer Review

SensiFAST SYBR No-ROX One-Step Kit Customer Review

 

  • Price
  • 318,00 €
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Dr. Monika Haas

Tel. +49 (0) 6221 714 15 16 info@biocat.com