Stable Cas9 HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor LocusDatasheet
|Cell Category:||Cas9 Expressing Cell Lines|
Premade HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor Locus
- Reliable, efficient genome-wide gRNA screens
- Simplified single or multiple gene knock-ins with Cas9 already active in cells
Stable AAVS1 Cas9 HEK293 Cell Line: High-expression of the Cas9 gene at the AAVS1 locus in HEK293 cells, as determined by qPCR (left panel). A Surveyor Assay of high expressing clones #8 and #17 shows that Cas9 is functional - arrow corresponds to band indicative of Cas9-mediated cleavage event, which is not present in untransfected cells.
With SBI's Cas9/HEK293 cells, you can go directly to transfection of your gRNAs and assessment of the biology - no need to spend time cloning Cas9 into the genome.
And when you need Cas9 expression from other cell types, you can turn to SBI's AAVS1 Cas9-gene donor vector or the corresponding kit including the AAVS1 gRNA/Cas9 vector in addition and integrate Cas9 into the AAVS1 safe harbor site in the cells of your choice - see links below.
Genetically modified organism (GVO), see General Terms