SARS-CoV-2 Additional Genome Coverage SNAP Panel (primers only)

99.7% Genomic Coverage! “If variants are there, you will see them”

User Manual

Product Description

Optimized NGS for SARS-CoV-2

Swift Normalase Amplicon SARS-CoV-2 NGS Panels (SNAP) enable identification of SARS-CoV-2 strains, including variants, from samples such as nasopharyngeal/oropharyngeal swabs, sputa, bronchoalveolar lavage (BAL), and stool.

  • 99.7% genomic coverage
  • Obtain genomes from viral titers as low as 10-100 viral copies
  • cDNA-to-sequencer in 3 hours
  • Up to 1536 UDIs

The SNAP SARS-CoV-2 Kit uses overlapping primers to generate 345 amplicons, sized 116-255 bp (average 150 bp), along the length of the 29.9 kb viral genome and obtain 99.7% coverage of the genome. Overlapping primers ensure that novel variants are detected, even when the mutation is a dropout of genomic sequence. Comprehensive mutation detection is crucial for tracking nucleotide variants and improve understanding of virus evolution, transmission, and pathogenesis.

 

Snap Sarscov2 280kreads 1mcopies Purple 600x178

Figure 1. Contiguous coverage of the SARS-CoV-2 genome from position 25 to 29853. 100,000 copies of gamma-irradiated SARS-CoV-2 (BEI, NR-52287) that were originally isolated from an oropharyngeal swab were converted into an NGS library with the Swift SNAP SARS-CoV-2 kit and sequenced 2 x 150 bp on an Illumina® MiniSeq® System. Resulting reads were downsampled to 280k reads per sample for analysis.

Obtain genomes from viral titers as low as 10-100 viral copies

10 to 1 million viral genome copies (Figure 2) are sufficient to generate NGS libraries using the SNAP SARS-CoV-2 panel. Mixed RNA samples were converted into first strand cDNA and used as input into the SNAP SARS-CoV-2 panel. Libraries were enzymatically normalized to 4nM using the Normalase workflow provided in the SNAP protocol.

 

Sensitive Ngs For Sars Cov 2

Figure 2. Obtain genomes from as few as 10-100 viral copies. SARS-CoV-2 synthetic template material (Twist Bioscience Cat. No. 102024) was mixed with UHR RNA (Agilent 740000) and converted into first-strand cDNA using the Superscript® IV First-Strand Synthesis System (Thermo Fisher 18091050). cDNA was converted into an NGS library with the Swift SNAP SARS-CoV-2 Kit and sequenced on the Illumina® MiniSeq® System at 2 x 150 bp. Resulting data was downsampled to 280k reads per sample.

cDNA-to-sequencer in 3 hours

Prepare first- or second-strand cDNA from samples such as Nasopharyngeal/Oropharyngeal Swabs, Sputa, Bronchoalveolar Lavage (BAL), Stool, or Wastewater (figure 3). Generate an NGS library using tiled primer pairs in a single tube to target the 29.9 kb viral genome. Primers were designed against the NCBI Reference Sequence NC_045512.2 (Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome) (Table 1).

Replace qPCR library quantification and normalization with the Normalase primers included in the kit. Produce consistent amplified library yields of 12 nM or 6 nM to generate equimolar pools and obtain balanced sample representation in sequencing.

Workflow

Figure 3. One-tube workflow prepares normalized libraries from cDNA in 3 hours by replacing qPCR library quantification with Normalase (included in the kit).

Swift also offers two other SARS-CoV-2 Primer Panels, the Swift Amplicon SARS-CoV-2 Panel and the SARS-CoV-2 S Gene Panel.

For Swift Normalase Amplicon Panel (SNAP) based NGS Target Enrichment Workflow please be sure to select one from each of the three categories to complete your order:

  1. Choose a SNAP Multiplex Primer Pool
  2. Add the the SNAP Core kit
  3. Choose a SNAP Indexing Primer Kit

All components have to be purchased individually.

  • Catalog Number
    COVG1V2-96-SWI
  • Supplier
    Swift Biosciences
  • Size
  • Shipping
    Dry Ice
  • Price
  • 1.188,00 €
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you need any help?

Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com