pPACK-SPIKE Delta (B.1.617.2), SARS-CoV-2 "S" Pseudotype - Delta (B.1.617.2) Variant - Lentivector Packaging Mix

User Manual

Product Description

Safely explore increased transmissibility and other features of the spike protein from the CDC variant of concern SARS-CoV-2 Delta (B.1.617.2)

  • Based on SBI’s popular and highly cited pPACKH1 Packaging System
  • Uses codon-optimized SARS-CoV-2 “S” protein from variant Delta (B.1.617.2, originally found in India) in place of VSV-G envelope protein
  • Spike protein mutations are T19R, G142D, ΔE156-157, R158G, L452R, T478K, D614G, P681R, D950N
  • Ideal for vaccine and antiviral efficacy studies under BSL2 conditions
  • Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
  • Compare with our full range of pPACK-SPIKE S protein pseudotyped lentiviruses to better understand emerging variants
  • Use with pPACK-BALD™, an envelope protein-free lentivector packaging system that is an ideal negative control


Potentially more transmissible than previous SARS-CoV-2 variants, the Delta variant (B.1.617.2) is classified as a variant of concern by the Centers for Disease Control (CDC) in the United States. This variant was initially observed in India and has since spread globally. With the pPACK-SPIKE™ Delta (B.1.617.2) Variant Spike Protein Lentivector Packaging Mix, you can safely characterize the SARS-CoV-2 S1 spike protein from lineage Delta (B.1.617.2), which contains the following mutations:

  • T19R
  • G142D
  • ΔE156-157
  • R158G
  • L452R
  • T478K
  • D614G
  • P681R
  • D950N

Designed to efficiently package most third-generation lentivectors, the pPACK-SPIKE Delta (B.1.617.2) Variant Spike Protein Lentivector Packaging Mix speeds and simplifies preparation of lentiviral particles pseudotyped with the SARS-CoV-2 T19R, G142D, ΔE156-157, R158G, L452R, T478K, D614G, P681R, D950N spike glycoprotein, making it an ideal reagent for vaccine and antiviral drug discovery projects.

Based on SBI’s highly cited pPACKH1 packaging system but with a truncated T19R, G142D, ΔE156-157, R158G, L452R, T478K, D614G, P681R, D950N Spike protein [1] replacing the standard VSV-G envelope protein, the pPACK-SPIKE Delta (B.1.617.2) Variant Spike Protein Packaging Mix consists of three plasmids that produce all of the structural and  replication proteins needed to transcribe and package an RNA copy of an expression lentivector into recombinant, T19R, G142D, ΔE156-157, R158G, L452R, T478K, D614G, P681R, D950N “Spike” pseudotyped lentiviral particles. As a result, you can conduct a range of SARS-CoV-2 studies under BSL-2 conditions, including neutralization assays, studies of virus interactions with host surface proteins, and the development of vaccines and therapeutics.

For added convenience we offer pPACK-BALD™, an envelope protein-free lentivector packaging mix that can be used as a negative control for any pPACK-SPIKE study, or for creating lentivirus particles pseudotyped with the envelope protein of your choice.

In addition, ACE2-expressing stable cell lines are provided.

pPACK-SPIKE Delta (B.1.617.2) Variant Spike Protein Lentivector Packaging Mix is available as stand-alone packaging mixes with sufficient plasmids for 10 reactions (standard size) or 25 reactions (XL), as well as the pPACK-SPIKE Beta Combo Kit that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution.

How It Works

pPACK-SPIKE is easy to use - simply co-transfect the pPACK-SPIKE Plasmids and your lentivector construct into 293TN producer cells or other lentiviral production cells, isolate pseudoviral particles with an optional concentration step, and conduct your studies.



Available pPACK-SPIKE S protein variants

Catalog Number Spike Variant WHO label  Spike Protein Mutation
CVD19-500A-1-SBI SARS-CoV-2 “S” Pseudotype   Original Wuhan-Hu-1 strain
CVD19-530A-1-SBI D614G Mutant    D614G
CVD19-560A-1-SBI N501Y Mutant    N501Y
CVD19-590A-1-SBI B.1.1.7 (UK) Variant  Alpha ΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
CVD19-580A-1-SBI B.1.351 (S.A.) RBD Mutations Beta K417N, E484K, & N501Y
CVD19-600A-1-SBI B.1.351 (S.A.) S1 Mutations (RBD+D614G) Beta K417N, E484K, N501Y, & D614G
CVD19-640A-1-SBI B.1.351 (S.A.) complete spike mutations Beta ΔH69/V70, Q52R, E484K, D614G, Q677H, F888L
CVD19-610A-1-SBI B.1.429 (CAL.20C) Variant  Epsilon  S13I, W152C, L452R, & D614G
CVD19-620A-1-SBI B.1.525 Variant  Eta ΔH69/V70, Q52R, E484K, D614G, Q677H, & F888L
CVD19-630A-1-SBI P1 Variant Gamma L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
CVD19-650A-1-SBI B.1.617.2 Variant Delta T19R, G142D, ΔE156-157, R158G, L452R, T478K, D614G, P681R, D950N
  • Catalog Number
    CVD19-650A-1-SBI
  • Supplier
    SBI System Biosciences
  • Size
  • Shipping
    Blue Ice
  • Price
  • 763,00 €
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