3-D Life Dextran-PEG Hydrogel FG Kit

The 3-D Life Dextran-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels.

User Manual

Product Description

Major components  of the 3-D Life Dextran-PEG Hydrogel Fast Gelling Kit are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001-CS) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

Application

3-D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Components

Material Quantity Concentration of reactive groups
Mal-Dextran, lyophilized 170 µl 30 mmol/L
PEG-Link, lyophilized 200 µl* 20 mmol/L*
10x CB (pH 5.5) 200 µl n.a.
Water 2 x 1500 µl n.a
*Volume/concentration after reconstitution of lyophilisate.


3-D Life
  Hydrogels can be set up with various compositions. For calculation of the volumes of components please use the Calculation Tool.

For more information about the complete 3-D Life Biomimetic Hydrogel System please refer to the 3-D Life Hydrogel User Guide.

  • Catalog Number
    FG90-1-CS
  • Supplier
    Cellendes GmbH
  • Size
  • Shipping
    RT
  • Price
  • 75,00 €
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Dr. Bettina Buchmann

Tel. +49 (0) 6221 71415 16 info@biocat.com