3-D Life Dextran-PEG Hydrogel FG Kit
The 3-D Life Dextran-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels.User Manual
Major components of the 3-D Life Dextran-PEG Hydrogel Fast Gelling Kit are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.
Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001-CS) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.
Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.
3-D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.
|Material||Quantity||Concentration of reactive groups|
|Mal-Dextran, lyophilized||170 µl||30 mmol/L|
|PEG-Link, lyophilized||200 µl*||20 mmol/L*|
|10x CB (pH 5.5)||200 µl||n.a.|
|Water||2 x 1500 µl||n.a|
|*Volume/concentration after reconstitution of lyophilisate.|
3-D Life Hydrogels can be set up with various compositions. For calculation of the volumes of components please use the Calculation Tool.
For more information about the complete 3-D Life Biomimetic Hydrogel System please refer to the 3-D Life Hydrogel User Guide.