3-D Life Dextran-CD Hydrogel FG Kit
The 3-D Life Dextran-CD Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels.User Manual
Major components of the 3-D Life Dextran-CD Hydrogel Fast Gelling Kit are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.
CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9 . It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.
Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001-CS) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.
Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.
|Material||Quantity||Concentration of reactive groups|
|Mal-Dextran, lyophilized||170 µl||30 mmol/L|
|CD-Link, lyophilized||200 µl*||20 mmol/L*|
|10x CB (pH 5.5)||200 µl||n.a.|
|Water||2 x 1500 µl||n.a|
|*Volume/concentration after reconstitution of lyophilisate.|
3-D Life Hydrogels can be set up with various compositions. For calculation of the volumes of components please use the Calculation Tool.
For more information about the complete 3-D Life Biomimetic Hydrogel System please refer to the 3-D Life Hydrogel User Guide.