BlasTaq™ HotStart DNA Polymerase

Eliminate non-specific amplification and primer-dimer formation

User Manual

Product Description

BlasTaq™ HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.

During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors.

Product Features

  • No non-specific amplification or primer-dimers
  • Improves yield of desired product
  • Simplifies reaction setup

 

G595 Blastaq Hotstart Dna Polymerase 2

G595 Blastaq Hotstart Dna Polymerase Data1 1 2

  • Catalog Number
    G595-ABM
  • Supplier
    ABM
  • Size
  • Shipping
    Blue Ice
  • Price
  • 326,00 €
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Dr. Monika Haas

Tel. +49 (0) 6221 71415 16 info@biocat.com