3-D Life PVA-PEG Hydrogel SG Kit

The 3-D Life PVA-PEG Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels.

User Manual

Product Description

Major components  of the 3-D Life PVA-PEG Hydrogel SG Kit are polymeric ­SG-PVA and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.

Prior to the crosslinking step, peptides (e.g. 3-D Life RGD Peptide, Cat.No. 09-P-001-CS) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix.

Components

Material Quantity Concentration of reactive groups
SG-PVA 170 µl 30 mmol/L
PEG-Link, lyophilized 200 µl* 20 mmol/L*
10x CB (pH 7.2) 200 µl n.a.
Water 2 x 1500 µl n.a
*Volume/concentration after reconstitution of lyophilisate.


3-D Life
  Hydrogels can be set up with various compositions. For calculation of the volumes of components please use the Calculation Tool.

For more information about the complete 3-D Life Biomimetic Hydrogel System please refer to the 3-D Life Hydrogel User Guide.

  • Catalog Number
    G82-1-CS
  • Supplier
    Cellendes GmbH
  • Size
  • Shipping
    RT
  • Price
  • 70,00 €
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you need any help?

Please contact:

Dr. Bettina Buchmann

Tel. +49 (0) 6221 71415 16 info@biocat.com