3-D Life PVA-CD Hydrogel SG Kit
The 3-D Life PVA-CD Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels.User Manual
Major components of the 3-D Life PVA-CD Hydrogel SG Kit are polymeric SG-PVA and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.
CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9 . It allows cells to spread and migrate within the hydrogel if they express the indicated MMPs. In most cases cell spreading and migration also requires the presence of adhesion peptides.
Prior to the cross-linking step, peptides (e.g. 3-D Life RGD Peptide, Cat.No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix.
|Material||Quantity||Concentration of reactive groups|
|SG-PVA||170 µl||30 mmol/L|
|CD-Link, lyophilized||200 µl*||20 mmol/L*|
|10x CB (pH 7.2)||200 µl||n.a.|
|Water||2 x 1500 µl||n.a|
|*Volume/concentration after reconstitution of lyophilisate.|
3-D Life Hydrogels can be set up with various compositions. For calculation of the volumes of components please use the Calculation Tool.
For more information about the complete 3-D Life Biomimetic Hydrogel System please refer to the 3-D Life Hydrogel User Guide.