3-D Life ToGro Hydrogel Kit
The 3-D Life ToGrow Hydrogel Kit contains a preformulated thiol-reactive polymer and a crosslinker. 3-D cell cultures can be set up by adding a cell suspension and the crosslinker to the thiol-reactive polymer.User Manual
The 3-D Life ToGro Hydrogel Kit contains reagents for the preparation of hydrogels with a soft stiffness (400 Pa; shear modulus) and a RGD Peptide concentration of 0.4 mmol/L. The gel composition allows three-dimensional spreading and migration of most cells.
The gel is formed by the crosslinking of modified dextran carrying the cell adhesion motif RGD (RGD-Dextran) with CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on RGD-Dextran resulting in gel formation within 20 min. The formation of hydrogel is performed at physiological pH for optimal cell compatibility. CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide. The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9 . It allows cells to spread and migrate within the gel if they express the indicated MMPs.
Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.
3-dimensional cultivation of various cell types, spheroids, tissue samples and similar requiring a cell-adhesive and cell-degradable matrix for cell growth and differentiation.
|Material||Quantity||Concentration of reactive groups|
|RGD-Dextran, lyophilized||2 x 870 µl*||n.a.|
|CD-Link, lyophilized||200 µl*||20 mmol/L*|
|Reconstitution Buffer||2 x 900 µl||n.a.|
|*Volume/concentration after reconstitution of lyophilisate.|