3-D Life Dextran-HA Hydrogel SG Kit

The 3-D Life Dextran-HA Hydrogel SG Kit provides reagents for setting up hydrogels containing hyaluronic acid.

User Manual

Product Description

Major components of the 3-D Life Dextran-HA Hydrogel SG Kit are SG-Dextran and the thiol-functionalized hyaluronic acid crosslinker HyLink. When SG-Dextran and HyLink are combined, thiol groups on HyLink form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001-CS) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix.

SG-Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

Application

3-D cell cultures, , hydrogel injections, filling of microchannels, generation of soft to very stiff gels.

Components

Material Quantity Concentration of reactive groups
SG-Dextran 170 µl 30 mmol/L
HyLink, lyophilized 2 x 200 µl* 10 mmol/L*
10x CB (pH 7.2) 200 µl n.a.
Water 2 x 1500 µl n.a
*Volume/concentration after reconstitution of lyophilisate.


3-D Life
  Hydrogels can be set up with various compositions. For calculation of the volumes of components please use the Calculation Tool.

For more information about the complete 3-D Life Biomimetic Hydrogel System please refer to the 3-D Life Hydrogel User Guide.

  • Catalog Number
    G95-1-CS
  • Supplier
    Cellendes GmbH
  • Size
  • Shipping
    RT
  • Price
  • 180,00 €
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Please contact:

Dr. Bettina Buchmann

Tel. +49 (0) 6221 71415 16 info@biocat.com