Pyruvate Assay KitUser Manual
|Research Area:||Cell Metabolism|
|Assay Category:||Tricarboxylic Acid Cycle/Krebs Cycle/Citric Acid Cycle|
|Sample Type:||Suitable for use with serum, plasma, biological samples, and lysates|
Our Pyruvate Assay Kit measures pyruvate in biological samples. First, pyruvate is oxidized by pyruvate oxidase, producing hydrogen peroxide. The hydrogen peroxide is then detected at ex. 530-570 nm/em. 590-600 nm using a specific fluorometric probe. Pyruvate levels in unknown samples are determined based on the provided pyruvate standard curve.