SARS-CoV-2 Nucleocapsid protein (Q384H), His Tag
Datasheet| Specifications | |
|---|---|
| Molecular Weight: | 47.3 kDa |
| Tag: | His Tag |
| Source: | SARS-CoV-2 Nucleocapsid protein (Q384H), His Tag (NUN-C52H4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (Q384H)). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutation Q384H was identified on the nucleocapsid protein of SARS-CoV-2 variants with high frequency (~7%) in variants circulating in Africa (data retrieved from https://pubmed.ncbi.nlm.nih.gov/33095454/). |
| Purity: | >90% as determined by SDS-PAGE. |
| Endotoxin Level: | Less than 1.0 EU per μg by the LAL method. |
| Format: | Powder |
| Additional Information | |
|---|---|
| Synonyms: | Nucleocapsid protein,NP,Protein N |
| Organism: | Other |
| Acc.No.: | QHO62115.1 (Q384H) |
Sequence Information
Met 1 - Ala 419
Background
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.