NileHiFi® Long Amplicon PCR Kit
High-fidelity DNA polymerases with strong proofreading activityData sheet
GeneCopoeia's NileHiFi® Long Amplicon PCR Kit allows you to amplify templates up to 18kb of human genomic DNA (gDNA), 30kb of λ phage DNA, and other high molecular weight genomic DNA. This kit is recommended for amplifying GC rich or complex targets. The NileHiFi® Long Amplicon PCR Kit is the ideal choice for generating long PCR products with high sensitivity and processivity.
- Efficiently amplify DNA templates up to 18 kb
- Compatible with blunt-end and TA cloning
- Includes all components required for PCR except primers and template
The NileHiFi® Long Amplification PCR Kit contains a hot-start antibody-modified polymerase that remains inactive before the first denaturation step. The polymerase has a proofreading function that allows mismatches incorporated at the 3′ end of the growing strand to be repaired. Our optimized PCR polymerase can effectively reduce the nonspecific amplification.