SensiMix SYBR & Fluorescein Kit

Outstanding assay reproducibility and sensitivity for both DNA and cDNA templates, under standard conditions.

  • Sensitive - reproducible detection of low-copy number templates
  • Reproducible – consistent results between technical replicates for increased confidence in results
  • Specific - proprietary hot-start modification minimizes non-specific amplification for improved assay reliability in high-throughput assays
  • Robust – reliable, accurate detection of DNA and cDNA targets from a broad range of sample types
user manual

Product Description

The SensiMix™ SYBR® & Fluorescein Kit has been developed for highly accurate real-time PCR and has been validated on real-time PCR platforms that require the passive reference dye fluorescein for collection of dynamic well factors.

A proprietary, highly-optimized, chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix SYBR® & Fluorescein Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions.

The SensiMix SYBR® & Fluorescein Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications

  • Gene expression analysis
  • DNA / cDNA target detection
  • miRNA profiling / quantification
  • Copy number variation (CNV) analysis

Broad dynamic range SensiMix SYBR & Fluorescein Kit

Amplification curves were obtained for the YWHAZ gene over 5 log dilution series of mouse cDNA. Reactions were in quadruplicate, using standard reaction conditions. The results demonstrate even spread of the curves with no inhibition even at the highest cDNA inputs.

  • Catalog Number
    QT615-05-BL
  • Supplier
    Meridian (Bioline)
  • Size
  • Shipping
    Blue Ice
  • Price
  • 761,00 €
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you need any help?

Please contact:

Dr. Monika Haas

Tel. +49 (0) 6221 714 15 16 info@biocat.com