293RTV Retroviral Packaging Cell LineData sheet
|Cell Category:||Packaging Cell Lines|
- Grows faster than the parental 293 cell line
- Produces higher yields of retrovirus
- Provides firmer attachment to culture plates
The 293RTV Cell Line is a permanent line established from primary embryonic human kidney transformed with human adenovirus type 5 DNA. The genes encoded by the E1 region of adenovirus (E1a and E1b) are expressed in these cells and participate in transactivation of viral promoters, allowing these cells to produce high levels of protein.
293RTV also stably expresses the SV40 large T antigen, through cloning and multiple rounds of testing for viral yield, 293RTV is specifically selected for high level of retroviral production.
Use the 293RTV Packaging Cell Line along with the pCMV-VSV-G and pCMV-Gag-Pol vectors and a cloning / expression vector for retrovirus production.
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Genetically modified organism (GVO), see General Terms