SARS-CoV-2 S Gene SNAP Panel (primers only)

Focused Insights for Faster and High Throughput Surveillance

User Manual

Product Description

Swift Normalase Amplicon SARS-CoV-2 S Gene NGS Panels (SNAP) utilize multiple overlapping amplicons in a single tube, 2-hour workflow to prepare ready-to-sequence libraries. The unique workflow generates robust libraries from as few as 10-100 viral copies or 50 ng of cDNA. Library quantification by qPCR or Qubit® may be omitted when using the Swift SNAP indexing primers.

Overlapping primer pairs used in SNAP generate libraries from first- or second-strand cDNA produced from viral isolates or clinical specimens. 384 Unique Dual Indexes (UDIs) for use on Illumina NGS systems are available now.

Sgene Cov 25kreads 210216 1024x152

Figure 1. The SARS-CoV-2 S Gene Panel provides 100% coverage of the gene which encodes for the SARS-CoV-2 spike protein. A library generated from 1 million copies of the BEI standard (NR-52285) was sequenced on an Illumina® MiniSeq® System. Results were down sampled to 25,000 reads per sample and aligned and visualized using the Integrated Genomics Viewer (IGV).

The Swift Normalase® Amplicon SARS-CoV-2 S GENE Panel (SNAP) is expertly designed for researchers who are targeting the S Gene to determine novel strains and the consequences of infectivity and transmissibility.

  • SARS-CoV-2 genomics coordinates covered: 21497-25421
  • Provides 100% coverage of the S Gene from limited viral titers
  • Generate amplicons by multiplex PCR in a single tube, 2-hour reaction
  • Supports high-throughput library quantification methods (fluorometric, electrophoretic, or Swift Normalase)
  • Optimized for all Illumina® sequencing platforms

Workflow

Figure 2. One-tube workflow prepares normalized libraries from cDNA in 3 hours by replacing qPCR library quantification with Normalase (included in the kit).

Swift also offers two other SARS-CoV-2 Primer Panels, the Swift Amplicon SARS-CoV-2 Panel and the SARS-CoV-2 Additional Primer Coverage Pool.

For Swift Normalase Amplicon Panel (SNAP) based NGS Target Enrichment Workflow please be sure to select one from each of the three categories to complete your order:

  1. Choose a SNAP Multiplex Primer Pool
  2. Add the the SNAP Core kit
  3. Choose a SNAP Indexing Primer Kit

All components have to be purchased individually.

 

  • Catalog Number
    SGENE-96-SWI
  • Supplier
    Swift Biosciences
  • Size
  • Shipping
    Dry Ice
  • Price
  • 850,00 €
Add to Cart
you need any help?

Please contact:

Dr. Sieke Schaepe

Tel. +49 (0) 6221 71415 16 info@biocat.com