SARS-CoV-2 S protein (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V) trimer, His Tag (MALS verified), Variant Lineage B.1.351
Datasheet| Specifications | |
|---|---|
| Molecular Weight: | 137.6 kDa |
| Tag: | His Tag |
| Source: | SARS-CoV-2 S protein trimer, His Tag (SPN-C52Hk) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P/ A892P/ A899P/ A942P/ K986P/ V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. The L18F/ D80A/ D215G/ LAL242-244del/ R246I/ K417N/ E484K/ N501Y/ D614G/ A701V mutations were identified in the SARS-CoV-2 Beta variant (Pango lineage: B.1.351; other names: 20H/501Y.V2). |
| Purity: | >90% as determined by SDS-PAGE. |
| Endotoxin Level: | Less than 1.0 EU per μg by the LAL method. |
| Format: | Powder |
| Additional Information | |
|---|---|
| Synonyms: | Spike,S protein,Spike glycoprotein,S glycoprotein |
| Organism: | Other |
| Acc.No.: | QHD43416.1 |
Sequence Information
Val 16 - Pro 1213
Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.