Biotinylated SARS-CoV-2 S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) trimer, His,Avitag™ (MALS verified)

Datasheet
Specifications
Molecular Weight: 139.8 kDa
Tag: His Tag & Avi Tag
Source: Biotinylated SARS-CoV-2 S protein trimer, His,Avitag (SPN-C82E6) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P/ A892P/ A899P/ A942P/ K986P/ V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. The L18F/ T20N/ P26S/ D138Y/ R190S/ K417T/ E484K/ N501Y/ D614G/ H655Y/ T1027I/ V1176F) mutations were identified in the SARS-CoV-2 Gamma variant (Pango lineage: P.1; other names: 20J/501Y.V3).
Purity: >95% as determined by SDS-PAGE.
Endotoxin Level: Less than 1.0 EU per μg by the LAL method.
Format: Powder
Additional Information
Synonyms: Spike,S protein,Spike glycoprotein,S glycoprotein
Organism: Other
Acc.No.: QHD43416.1

Sequence Information

Val 16 - Pro 1213

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

  • Catalog Number
    SPN-C82E6-25ug-AC
  • Supplier
    ACROBiosystems
  • Size
  • Shipping
    RT
  • Price
  • 658,00 €
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