OxiSelect™ BPDE Protein Adduct ELISA KitUser Manual
|Research Area:||Oxidative Stress|
|Assay Category:||Protein Oxidation Assays|
|Sample Type:||Suitable for use with protein samples such as purified protein, plasma, serum, or cell lysates|
BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC. The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards.
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