OxiSelect™ UV-Induced DNA Damage ELISA Kit (CPD Quantitation)User Manual
|Research Area:||Oxidative Stress|
|Assay Category:||DNA/RNA Damage and Repair Assays|
The OxiSelect Oxidative UV-induced DNA Damage ELISA Kit (CPD Quantitation) is an enzyme immunoassay developed for rapid detection and quantitation of CPD in any DNA samples. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
CDP-DNA standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA high-binding plate. The CPDs present in the sample or standard are probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody. The CPD content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined CPD-DNA standards.
- Cui, H. et al. (2016) The Stress-responsive Gene ATF3 mediates dichotomous UV responses by regulating the Tip60 and p53 proteins. J Biol Chem.
- Donninger, H. et al. (2015) The RASSF1A Tumor Suppressor Regulates XPA-Mediated DNA repair. Mol Cell Biol.
- Rimann, M. et al. (2015) Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells. J Lab Autom.
- Zirkin, S. et al. (2013) The PIM-2 Kinase Is an Essential Component of the Ultraviolet Damage Response That Acts Upstream to E2F-1 and ATM. J Biol.
- Burgess, H.M. et al. (2011) Nuclear Relocalisation of Cytoplasmic poly(A)-binding Proteins PABP1 and PABP4 in Response to UV Irradiation Reveals mRNA-dependent Export of Metazoan PABPs. J Cell Sci