OxiSelect™ Cellular UV-Induced DNA Damage Staining Kit (CPD)

User Manual
Research Area: Oxidative Stress
Assay Category: DNA/RNA Damage and Repair Assays
Detection Method: Fluorescence Microscopy

Product Description

The OxiSelect Cellular UV-induced DNA Damage Staining Kit (CPD) is an immunofluorescence assay developed for rapid detection of CPDs in genomic DNA of cultured cells. Each kit provides sufficient reagents for up to 96 stainings in a 96-well plate.

Assay Principle

Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing CPD damage are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

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  • Supplier
    Cell Biolabs
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  • Price
  • 618,00 €
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