OxiSelect™ Superoxide Dismutase Activity Assay, Trial Size

User Manual
Research Area: Oxidative Stress
Assay Category: Superoxide Dismutase Assay
Sample Type: Suitable for use with plasma, serum, cells or tissues
Detection Method: Colorimetric

Product Description

The OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions. The included chromagen produces a water-soluble formazan dye upon reduction by superoxide anions. The activity of SOD is determined as the inhibition of chromagen reduction.

The Superoxide Dismutase Activity Assay is a fast and reliable kit for the measurement of SOD activity from cell lysate, plasma, serum, tissue homogenates. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, SOD standards and unknown protein samples.

Assay Principle

Superoxide anions (O2-) are generated by a Xanthine/Xanthine Oxidase (XOD) system, and then detected with a Chromagen Solution. However, in the presence of SOD, these superoxide anion concentrations are reduced, yielding less colorimetric signal.

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