OxiSelect™ 96-Well Comet Assay KitUser Manual
|Research Area:||Oxidative Stress|
|Assay Category:||DNA/RNA Damage and Repair Assays|
|Detection Method:||Fluorescence Microscopy|
- Useful screening tool for various types of DNA damage
- Slides are specially treated for adhesion of low-melting agarose
- Easy visualization by epifluorescence microscopy
The OxiSelect 96-Well Comet Assay is a single cell gel electrophoresis assay (SCGE) for simple evaluation of cellular DNA damage. First, individual cells are mixed with molten agarose before application to the OxiSelect 96-Well Comet Slide. These embedded cells are then treated with a lysis buffer and alkaline solution, which relaxes and denatures the DNA. Finally, the samples are electrophoresed in a horizontal chamber to separate intact DNA from damaged fragments. Following electrophoresis, the samples are dried, stained with a DNA dye, and visualized by epifluorescence microscopy. Under these conditions, the damaged DNA (containing cleavage and strand breaks) will migrate further than intact DNA and produce a “comet tail" shape. Each kit provides sufficient reagents to perform up to 96 assays.
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