OxiSelect™ N-epsilon-(carboxyethyl) lysine (CEL) Competitive ELISA KitUser Manual
|Research Area:||Oxidative Stress|
|Assay Category:||Advanced Glycation End (AGE) Products Assays|
- Provides rapid detection and quantitation of CEL protein adducts
- Perform up to 96 assays, including standard curve and unknown protein samples
- Will not cross react with CML protein adducts
First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect™ CEL ELISA Kit will not cross react with CML protein adducts.
- Garg D, et al. (2017) Correlation between follicular fluid levels of sRAGE and vitamin D in women with PCOS. J Assist Reprod Genet. doi: 10.1007/s10815-017-1011-6