OxiSelect N-epsilon-(carboxymethyl) lysine (Cml) Competitive ELISA KitUser Manual
|Research Area:||Oxidative Stress|
|Assay Category:||Advanced Glycation End (AGE) Products Assays|
|Sample Type:||Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples|
- Detect CML as low as 50 ng/mL from a variety of samples
- CML-modified BSA included as standard
- Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples
The OxiSelect™ Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts. The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.
First, a CML conjugate is coated on the ELISA plate. The unknown CML protein samples or CML-BSA standards are then added to the CML conjugate preabsorbed plate. After a brief incubation, the anti-CML monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CML protein adducts in unknown samples is determined by comparison with the predetermined CML-BSA standard curve.
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