OxiSelect N-epsilon-(carboxymethyl) lysine (Cml) Competitive ELISA Kit

User Manual
Specifications
Research Area: Oxidative Stress
Assay Category: Advanced Glycation End (AGE) Products Assays
Sample Type: Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples
Detection Method: Colorimetric

Product Description

  • Detect CML as low as 50 ng/mL from a variety of samples
  • CML-modified BSA included as standard
  • Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples

The OxiSelect™ Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts. The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.

Assay Principle:

First, a CML conjugate is coated on the ELISA plate. The unknown CML protein samples or CML-BSA standards are then added to the CML conjugate preabsorbed plate. After a brief incubation, the anti-CML monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CML protein adducts in unknown samples is determined by comparison with the predetermined CML-BSA standard curve.

Product Citations

  • Price
  • 765,00 €
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