EPHA5 Stable Ba/F3 Cell Line
|Cell Category:||Drug Discovery Cell Lines|
|Tissue of Origin:||Bone Marrow|
|Expressed Gene:||EPHA5 tyrosine kinase (GFP-ETV6-EPHA5) via retroviral transduction|
Fusing a kinase domain to a homodimerization domain such as the PNT domain of ETV6 can result in activated kinase. The EPHA5 Stable Ba/F3 Cell Line expresses activated EPHA5 tyrosine kinase that is linked to a GFP reporter (GFP-ETV6-EPHA5 in MSCV-puro). Ba/F3 cells expressing activated tyrosine kinase become completely dependent on the kinase activity for survival and as a result, the cells can be propagated even in the absence of IL-3.
EPHA5 belongs to the EPH family of receptor tyrosine kinases consisting of proteins which bind to ephrin. These proteins are expressed in nearly all tissues during embryo development, and they are involved in a variety of developmental processes which include formation of the nervous system, cardiovascular system, and axon guidance. More recently, they have been implicated in adult biological functions.
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Genetically modified organism (GVO), see General Terms