EPHA8 Stable Ba/F3 Cell Line
|Cell Category:||Drug Discovery Cell Lines|
|Tissue of Origin:||Bone Marrow|
|Expressed Gene:||EPHA8 tyrosine kinase (GFP-ETV6-EPHA8) via retroviral transduction|
Fusing a kinase domain to a homodimerization domain such as the PNT domain of ETV6 can result in activated kinase. The EPHA8 Stable Ba/F3 Cell Line expresses activated EPHA8 tyrosine kinase that is linked to a GFP reporter (GFP-ETV6-EPHA8 in MSCV-puro vector). Ba/F3 cells expressing activated tyrosine kinase become completely dependent on the kinase activity for survival and as a result, the cells can be propagated even in the absence of IL-3.
EPHA8 belongs to the EPH family of receptor tyrosine kinases consisting of proteins which bind to ephrin. These proteins are expressed in nearly all tissues during embryo development, and they are involved in a variety of developmental processes which include formation of the nervous system, cardiovascular system, and axon guidance. EPHA8 functions as a receptor for ephrin A2, A3 and A5 and plays a role in short-range contact-mediated axonal guidance during development of the mammalian nervous system.
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Genetically modified organism (GVO), see General Terms