VitroGel® STEM is a xeno-free hydrogel system developed to improve the performance of three-dimensional (3D) static suspension cultures and scale-up of human pluripotent stem cells (hPSCs) to create a high-throughput system to model various tissue and disease states.User Manual Brochure
This hydrogel system is ready-to-use with an optimized formulation that fully supports the rapid expansion of high-quality 3D stem cell spheroids with pluripotent properties. hPSCs directly thawed from liquid nitrogen or passaged from 2D matrix coated culture vessels can be immediately mixed with the hydrogel solution for static suspension cultures. Moreover, the optimization protocol is ideal for time-sensitive experiments, as it does not require excessive medium exchanges, which can ultimately save on time and materials. This hydrogel system is compatible with most hPSC culture media and tissue culture vessels. Due to the unique static suspension culture procedure, the requirement for microcarriers for large-scale bioreactors is eliminated, making the cell harvesting simple and effective. Cells cultured in this system can be harvested out with the VitroGel Cell Recovery Solution. The 3D stem cell spheroids that are developed using this system can be used for further sub-culturing, patterned differentiating, organoid developing, or re-establishing 2D culture morphologies.
- 3D static suspension culture for hPSCs
- Work at room temperature
"Just add cells" 5 min protocol
No matrix coating required. VitroGel STEM is ready-to-use. Just mix with your hPSCs. There is no laborous matrix coating required to maintain and expand your stem cells.