ViraSafe™ Lentiviral Expression System (Puro), EcotropicUser Manual
ViraSafe Universal Lentiviral Expression System
- Transfer Plasmid: Reduce extent of HIV sequences to increase capability up to 10 kb and reduce likelihood of recombination between vector components. Add elements to increase titer and further improve safety
- Packaging Plasmid: Improve the packaging plasmid to increase performance and reduce the likelihood of recombination between vector components
a. Minimize HIV sequences – no accessory proteins, Tat or Rev, or LTRs
b. Prevent overlap with vector SM by codon wobbling Gag sequences
c. Boost particle production by incorporating adenovirus VAI element
- Flexible: All vectors including packaging vectors are provided separately to allow end-user to optimize the vector ratio for maximal lentivirus production
Cell Biolabs’ ViraSafe™ Lentiviral Expression System provides a much safer method to package lentivirus, while still providing high viral titers. The sequence homology with native HIV-1 has been reduced by 80-90% even compared with commercial third-generation packaging systems. In addition, each plasmid is provided separately rather than in a packaging mixture. This allows you the flexibility to amplify individual plasmids and optimize the ratio of plasmids for your experiment.
pSMPUW-Puro Lentiviral Expression Vector contains EF-1a promoter ahead of the multiple cloning sites, followed by PGK promoter and puromycin resistant gene.
pSMPUW-Puro Lentiviral Expression Vector (6145 bp, Kanamycin-resistant)