ViraSafe™ Lentiviral Expression System (Hygro), Ecotropic

User Manual

Product Description

ViraSafe Universal Lentiviral Expression System

  • Transfer Plasmid: Reduce extent of HIV sequences to increase capability up to 10 kb and reduce likelihood of recombination between vector components. Add elements to increase titer and further improve safety
  • Packaging Plasmid: Improve the packaging plasmid to increase performance and reduce the likelihood of recombination between vector components

a. Minimize HIV sequences – no accessory proteins, Tat or Rev, or LTRs

b. Prevent overlap with vector SM by codon wobbling Gag sequences

c. Boost particle production by incorporating adenovirus VAI element

  • Flexible: All vectors including packaging vectors are provided separately to allow end-user to optimize the vector ratio for maximal lentivirus production

Cell Biolabs’ ViraSafe™ Universal Lentiviral Expression System provides a much safer method to package lentivirus, while still providing high viral titers. The sequence homology with native HIV-1 has been reduced by 80-90% even compared with commercial third-generation packaging systems.

In addition, each plasmid is provided separately rather than in a packaging mixture. This allows you the flexibility to amplify individual plasmids and optimize the ratio of plasmids for your experiment.

pSMPUW-Hygro Lentiviral Expression Vector contains EF-1a promoter ahead of the multiple cloning sites, followed by PGK promoter and hygromycin resistant gene.

pSMPUW-Hygro Lentiviral Expression Vector (6568 bp, Kanamycin-resistant)

Packaging Plasmids

  • Catalog Number
    VPK-214-ECO-CB
  • Supplier
    Cell Biolabs
  • Size
  • Shipping
    Blue Ice
  • Price
  • 1.106,00 €
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you need any help?

Please contact:

Dr. Monika Haas

Tel. +49 (0) 6221 71415 16 info@biocat.com